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In 1866, he then demonstrated the similarity between Amphioxus [=Branchiostoma] and the larval stages of tunicates and established the chordate status of the tunicates.
In 1867, Kovalevsky extended the germ layer concept of Christian Heinrich Pander and Karl Ernst von Baer to include the invertebrates, establishing an important embryologic unity in the animal kingdom. image From Today In Science History